Application

model system to study the pathophisiology of rheumatoid arthritis, matrix mineralization, effects of inflammatory mediators, bone morphogenic proteins, bone remodeling and metabolism

Cell Line Origin

Bone

Components

Basal Medium containing 10% FBS & 10% DMSO

General description

Human Osteoblasts-Rheumatoid Arthritis, HOb-RA, are isolated from bone of patients with rheumatoid arthritis (RA) and provide an excellent model system to study the patophisiology of that disease in vitro, including matrix mineralization, effects of inflammatory mediators and bone morphogenic proteins, physiological control of bone remodeling and regulation of bone metabolism.Characterization: Positive for bone mineralization.

Preparation Note

2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO Can be cultured at least 10 doublings

Subculture Routine

Please refer to the HOb-RA Culture Protocol.